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Related post: size) and fluorescence in two independent channels at flow rates up to 5000 cells per second. Single parameter analysis, typically using fluorescein conjugated reagents, has already become a fairly standard immunologic tech- nique. The goal of the NIAID FACS installation is to provide as extensive collaborative opportunities as possible to investigators in the Institute as well as to explore new applications of this advanced technology. To this end a number of enhancements have been made in the computer software used with the FACS. Completely new programs have been written in a much more flexible and powerful computer operating system to collect and analyse FACS data. These store a complete description of the material being tested, staining agent(s), etc., and make it available during data retrieval. Exact operating parameters of the instrument are captured automatically allowing the display of fluores- cence in terms of molecules of fluorochrome or of conjugated ligand. Each investigator's data is automatically Xalatan .005 archived for rapid retrieval. Data evalu- ation and plotting can be set up to proceed automatically for large numbers of f i les. To date, twenty-two investigators have used the system. Some of the collabo- rative projects underway include: 1. The use of Cost of Xalatan charge sensitive cyanine dyes to measure the membrane potential of lymphocytes and their response to various stimuli. 2. The purification of human eosinophils to 98+^ based on their spontaneous autof luorescence. Since the Buy Xalatan 0.005 cells are unstained they function normally in several assays. 3. The study of spontaneous antibody to various types of murine leukemia viruses by indirect immunofluorescence. k. The study of murine B cell tumors for evidence of response to B eel 1 mi togens. 5. The isolation and cloning from mouse-hamster somatic cell hybrids of lines expressing different and distinct amounts of murine leukemia virus gp70 in an effort to map the responsible gene(s). 6. The development of a panel of rat anti-NZB spleen hybridomas. An attempt will be made to use the FACS in cloning them. The only additional wavelength of laser illumination suitable for the FACS is 568 nm. This is only 5 nm below the peak emission of TRITC. For this reason, in collaboration with Marvin Sternfeld of Research Organics, Cleveland, 2i»-63 Project No. ZOl Al 00203-01 LMI Ohio, a new dye, XRITC, was developed, which emits at 601 nm. Xalatan Coupon This dye serves well in two-color immunofluorescence with the FACS and is currently in use. A number of studies utilizing the two-color immunofluorescence are in progress. Software has been developed to measure emission anisotropy, a measure of membrane fluidity, which may correlate with lymphocyte activation. In the near future we expect to develop the hardware and software to allow simul- taneous immunofluorescence and measurement of fluorescence depolarization in order to begin the study of the relationship of membrane properties with surface differentiation antigens. Publ ications: Chused, T. M. , S. 0. Sharrow, J. Buy Xalatan Weinstein, W. J. Ferguson, and M. Sternfeld; XRITC: A new dye for two-color immunofluorescence. J. Histochem. Xalatan 0.005 and Cytochem. Xalatan Canada I98O. (In press) 24-64 SMITHSONIAN SCIENCE INFORMATION EXCHANGE PROJECT NUMBER Xalatan Price (Oo MOT ust this space J U.S. DEPARTMENT OF PROJECT NUMBER HEALTH, EDUCATION, AND WELFARE PUBLIC HEALTH, SERVICE Z01 Al 0020^-01 LM I NOTICE OF INTRAMURAL RESEARCH PROJECT PERIOD COVERED October 1, 1979 to September 30, 1980 TITLE Of PROJECT (80 characters or less) Immune Responses to Non-H-2 Antigens NAMES, UBORATORY AND INSTITUTE AFFILIATIONS, AND TITLES OF PRINCIPAL INVESTIGATORS AND ALL OTHER PROFESSIONAL PERSONNEL Xalatan Cost ENGAGED ON THE PROJECT PI: OTHER: W. F. Davidson H. C. Morse I 1 1 M. B. C. Dunlop'' T. M. Chused B. A. Taylor** Visiting Fellov* Senior Investigator Senior Investigator LMI/NIAID LMI/NIAID LMI/NIAID COOPERATING UNITS (if any) *John Curtin School of Medicine, Canberra, Australia **The Jackson Laboratory, Bar Harbor, Maine lab/ BRANCH Laboratory of Microbial Immunity SECT I ON Virology and Cellular Immunology INSTITUTE AND LOCATION NIAID, NIH, Bethesda, Maryland 20205 TOTAL MANYEARS: 1.2 PROFESSIONAL: 1.2 OTHER: CHECK APPROPRIATE BOX(ES) D (a) HUMAN SUBJECTS
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